单个动脉粥样硬化斑块的脂质分析和脂肪酸谱

Lipid analysis and fatty acid profiles of individual arterial atherosclerotic plaques.

作者信息

McGrath L T, Elliott R J

机构信息

Division of Biochemistry, School of Biology and Biochemistry, Queen's University of Belfast, Northern Ireland, United Kingdom.

出版信息

Anal Biochem. 1990 Jun;187(2):273-6. doi: 10.1016/0003-2697(90)90456-j.

DOI:10.1016/0003-2697(90)90456-j

PMID:2382829

Abstract

A protocol for the analysis of the lipid profile of microsamples of aortic tissue was developed. Lipid extraction was from intact tissue using acetone and chloroform/methanol (2/1, v/v). The extract was analyzed for total lipid, esterified cholesterol, cholesterol, triacylglycerol, and phospholipid. The extract was then processed to separate cholesteryl esters, triacylglycerol, and phospholipid which were hydrolyzed and the fatty acid composition was determined by GLC of pentafluorobenzyl ester derivatives. A lipid profile could be obtained on samples with a wet weight of 5 mg.

摘要

开发了一种用于分析主动脉组织微量样本脂质谱的方案。使用丙酮和氯仿/甲醇（2/1，v/v）从完整组织中提取脂质。对提取物进行总脂质、酯化胆固醇、胆固醇、三酰甘油和磷脂分析。然后对提取物进行处理，以分离胆固醇酯、三酰甘油和磷脂，将它们水解，并通过五氟苄基酯衍生物的气相色谱法测定脂肪酸组成。对于湿重为5毫克的样本可以获得脂质谱。