Catchatourian R, Eckerling G, Fried W
Blood. 1980 Apr;55(4):625-8.
To ascertain the effects of protein deprivation on hemopoietic parameters in otherwise healthy subjects, three volunteers were placed on diets containing 0.15 g protein/kg body weight for 8 days followed in 2 mo by another 8-day study period during which they ingested their usual diets containing more than 0.9 g protein/kg body weight. Complete blood counts, serum protein determinations, and tests of in vitro and in vivo leukocyte chemotaxis were performed prior to and at the conclusion of each study period. Subjects were phlebotomized of 500 ml on day 7 of each study period. Twenty-four-hour urinary erythropoietin excretion rates were assayed just prior to and again postphlebotomy. Reticulocyte counts were performed at intervals up to 1 wk postphlebotomy. Some of these determinations were replicated during a subsequent study. The hemoglobin and hematocrits decrased slightly but significantly after 8 days on low protein diets. Erythropoietin excretion rates and reticulocyte responses to phlebotomy were also less marked while subjects were on protein depleted diets. Leukocyte chemotaxis, measured both in vitro and in vivo, was also markedly reduced while subjects were on protein-depleted diets. We conclude that 8 days of moderately severe protein deprivation significantly impairs erythropoiesis and leukocyte function in otherwise healthy individuals.
为确定蛋白质缺乏对健康受试者造血参数的影响，三名志愿者食用含0.15克蛋白质/千克体重的饮食8天，随后在2个月内进行另一个为期8天的研究期，在此期间他们摄入通常的含超过0.9克蛋白质/千克体重的饮食。在每个研究期开始前和结束时进行全血细胞计数、血清蛋白测定以及体外和体内白细胞趋化性测试。在每个研究期的第7天对受试者进行500毫升静脉采血。在静脉采血前和采血后再次测定24小时尿促红细胞生成素排泄率。在静脉采血后长达1周的时间内定期进行网织红细胞计数。其中一些测定在随后的研究中重复进行。在低蛋白饮食8天后，血红蛋白和血细胞比容略有下降但具有统计学意义。在受试者食用蛋白质缺乏饮食期间，促红细胞生成素排泄率和网织红细胞对静脉采血的反应也不明显。在体外和体内测量的白细胞趋化性在受试者食用蛋白质缺乏饮食时也明显降低。我们得出结论，8天的中度严重蛋白质缺乏会显著损害健康个体的红细胞生成和白细胞功能。
