关于从生物提取物中制备高纯度过敏反应迟缓反应物质（SRS-A）
On the preparation of highly purified slow reacting substance of anaphylaxis (SRS-A) from biological extracts.
作者信息
Blackwell G J, Burka J F, Flower R J, Torkington P
出版信息
Br J Pharmacol. 1980 Jan;68(1):33-46. doi: 10.1111/j.1476-5381.1980.tb10696.x.
Abstract
1 Very highly purified (greater than 100,000 u/mg) slow reacting substance of anaphylaxis (SRS-A) has been prepared by reversed phase high pressure liquid chromatographic (HPLC) techniques. 2 High resolution liquid chromatography suggests that SRS-A may exist in at least three distinct forms which are possible tautomeric. 3 SRS-A collected by antigen challenge in vivo and by calcium ionophore-induced release in vitro are chromatographically indistinguishable. 4 Treatment of SRS-A with diazomethane but not sodium borohydride results in a loss of biological activity but treatment of the methyl ester with base results in a partial recovery of activity. 5 Highly purified SRS-A was examined by infrared and ultra-violet spectroscopy, and found to have a benzene-aromatic and probably an amino acid.
摘要
- 通过反相高压液相色谱（HPLC）技术制备了非常高纯度（大于100,000单位/毫克）的过敏反应慢反应物质（SRS-A）。2. 高分辨率液相色谱表明，SRS-A可能以至少三种不同形式存在，这些形式可能是互变异构体。3. 通过体内抗原激发和体外钙离子载体诱导释放收集的SRS-A在色谱上无法区分。4. 用重氮甲烷而非硼氢化钠处理SRS-A会导致生物活性丧失，但用碱处理甲酯会导致部分活性恢复。5. 用红外和紫外光谱对高纯度SRS-A进行了检测，发现其含有苯环芳烃，可能还含有一种氨基酸。
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