Drummond A H, Bucher F, Levitan I B
Brain Res. 1980 Feb 17;184(1):163-77. doi: 10.1016/0006-8993(80)90595-8.
The distribution of receptors for serotonin and dopamine has been studied in various neuronal and non-neuronal tissues from Aplysia californica using: (1) a [3H]LSD binding assay; and (2) stimulation of adenylate cyclase activity. High levels of specific [3H]LSD binding were found in all ganglia and nerves examined. Lower levels of binding were present in a number of muscle tissues and in the sheath surrounding the central ganglia. The ability of serotonin and dopamine to inhibit [3H]LSD binding depended upon the tissue examined. In muscle tissue, most of the binding was sensitive to serotonin. In contrast, a number of ganglia (e.g. the pleural, abdominal or cerebral) contained a considerable proportion of dopamine-sensitive binding. A limited pharmacological analysis of serotonin-sensitive [3H]LSD binding indicated that Aplysia serotonin receptors are closely related to those found in the snail, Helix pomatia, and in rat brain. Adenylate cyclase activity in membranes from Aplysia ganglia, muscles and connective nerves was stimulated by serotonin (but not by dopamine). The amount of serotonin-sensitive adenylate cyclase correlated well with the amount of serotonin-sensitive [3H]LSD binding in most tissues. D-LSD was a partial agonist on the serotonin-sensitive adenylate cyclase, whereas the pharmacologically inactive stereoisomer L-LSD was without effect. The high density of serotonin receptors in pleuro-abdominal connective nerves, and their presence in the connective tissue sheaths surrounding the ganglia, suggests that not all of these receptors are located at synapses. On the other hand, the tissue distribution of dopamine and serotonin receptors, as measured by these techniques, is consistent with that expected from electrophysiological data.
利用以下方法对加州海兔不同神经元及非神经元组织中5-羟色胺和多巴胺受体的分布进行了研究：（1）[3H]麦角酰二乙胺结合试验；（2）腺苷酸环化酶活性的刺激。在所检查的所有神经节和神经中均发现了高水平的特异性[3H]麦角酰二乙胺结合。在一些肌肉组织以及中枢神经节周围的鞘中，结合水平较低。5-羟色胺和多巴胺抑制[3H]麦角酰二乙胺结合的能力取决于所检查的组织。在肌肉组织中，大部分结合对5-羟色胺敏感。相比之下，一些神经节（如胸膜、腹部或脑）含有相当比例的对多巴胺敏感的结合。对5-羟色胺敏感的[3H]麦角酰二乙胺结合的有限药理学分析表明，海兔5-羟色胺受体与在蜗牛（苹果螺）和大鼠脑中发现的受体密切相关。海兔神经节、肌肉和结缔神经膜中的腺苷酸环化酶活性受到5-羟色胺的刺激（但不受多巴胺的刺激）。在大多数组织中，对5-羟色胺敏感的腺苷酸环化酶的量与对5-羟色胺敏感的[3H]麦角酰二乙胺结合的量密切相关。D-麦角酰二乙胺是对5-羟色胺敏感的腺苷酸环化酶的部分激动剂，而无药理学活性的立体异构体L-麦角酰二乙胺则无作用。胸腹结缔神经中5-羟色胺受体的高密度以及它们在神经节周围结缔组织鞘中的存在表明，并非所有这些受体都位于突触处。另一方面，通过这些技术测量的多巴胺和5-羟色胺受体的组织分布与电生理数据预期的一致。
