Kang M S, Song C W, Levitt S H
Cancer Res. 1980 Apr;40(4):1130-5.
The response of SCK tumor cells in vivo and in vitro to heat was compared, and the relationship between the kinetics of cell death and vascular function in tumors in vivo after hyperthermia was studied. The number of clonogenic cells in tumors excised immediately after heating was significantly less than that in the in vitro culture treated with the same heat doses. This suggested that the tumor cells in vivo are far more sensitive to direct damage by heat than are the cells in vitro. When the tumors were left in situ after hyperthermia at 43.5 degrees for 30 min, there was a progressive decrease in cell survival until 6 to 12 hr after the heating. The study of intravascular volume using the 51Cr-labeled red blood cell method indicated that severe vascular occlusion occurs in the tumor after hyperthermia. It therefore appeared that delayed cell death in tumors in vivo after hyperthermia resulted from an insufficient supply of oxygen and nutrients and an increase in acidity due to the vascular occlusion. Both the direct damage to tumor cells and the indirect damage to tumor cells as a consequence of vascular occlusion may play important roles in the eradication of tumors by hyperthermia.
比较了SCK肿瘤细胞在体内和体外对热的反应，并研究了热疗后体内肿瘤细胞死亡动力学与血管功能之间的关系。加热后立即切除的肿瘤中克隆形成细胞的数量明显少于接受相同热剂量体外培养的细胞数量。这表明体内肿瘤细胞对热直接损伤的敏感性远高于体外细胞。当肿瘤在43.5摄氏度热疗30分钟后留在原位时，细胞存活率逐渐下降，直至加热后6至12小时。使用51Cr标记红细胞法对血管内体积的研究表明，热疗后肿瘤内发生严重的血管闭塞。因此，热疗后体内肿瘤细胞的延迟死亡似乎是由于血管闭塞导致氧气和营养供应不足以及酸度增加所致。肿瘤细胞的直接损伤和血管闭塞导致的肿瘤细胞间接损伤可能在热疗根除肿瘤中起重要作用。
