Gottesman I S, Gerich J E
Clin Physiol Biochem. 1985;3(1):23-8.
Recovery and chromatography profiles of human plasma somatostatin extracted by three different procedures were compared. Extraction of plasma using acid-acetone-ether and octadecylsilyl silica gave comparable recovery (84 +/- 1 and 93 +/- 2%, respectively), which were nearly twofold greater than recovery achieved with the acid-ethanol method (50 +/- 1%, p less than 0.01). Acid-acetone-ether and octadecylsilyl silica extraction eliminated nearly completely immunoreactive material other than somatostatin-14 and -28, whereas the acid-ethanol-ether method did not. Because of its simplicity, octadecylsilyl silica is probably the method of choice for extraction of somatostatin from human plasma.
比较了通过三种不同方法提取的人血浆生长抑素的回收率和色谱图。使用酸 - 丙酮 - 乙醚和十八烷基硅烷硅胶提取血浆得到了相当的回收率（分别为84±1%和93±2%），这几乎是用酸 - 乙醇法获得的回收率（50±1%，p＜0.01）的两倍。酸 - 丙酮 - 乙醚和十八烷基硅烷硅胶提取几乎完全消除了除生长抑素 - 14和 - 28以外的免疫反应性物质，而酸 - 乙醇 - 乙醚法没有。由于其简便性，十八烷基硅烷硅胶可能是从人血浆中提取生长抑素的首选方法。
