1-甲氧基-5-甲基吩嗪硫酸甲酯（1-甲氧基吩嗪硫酸甲酯，1-methoxyPMS）在某些具有诊断重要性的酶测定中的应用。

Use of 1-methoxy-5-methylphenazinium methyl sulfate (1-methoxyPMS) in the assay of some enzymes of diagnostic importance.

作者信息

Nakamura S, Arimura K, Ogawa K, Yagi T

出版信息

Clin Chim Acta. 1980 Feb 28;101(2-3):321-6. doi: 10.1016/0009-8981(80)90260-0.

Abstract

1-Methoxy-5-methylphenazinium methyl sulfate (1-methoxyPMS) is a photochemically stable electron mediator between NAD(P)H and tetrazolium dyes. We have examined the efficiency of 1-methoxyPMS as an electron mediator in the assay of human lactate dehydrogenase (LDH, EC 1.1.1.27) and the activity staining of LDH isozymes after electrophoresis. The turnover number of 1-methoxyPMS as an electron mediator between NADH and nitrotetrazolium blue was 10--12 s-1, which is a value equal to that of PMS. Correlation coefficient between the estimated LDH activities of human sera obtained with 1-methoxyPMS and those obtained with diaphorase was 0.998. 1-MethoxyPMS successfully substituted for the unstable PMS in the activity staining of LDH isozymes of human serum, giving less background staining. We conclude that 1-methoxyPMS will be useful for routine methods of activity assay and activity staining of enzymes of diagnostic importance.

摘要

硫酸1-甲氧基-5-甲基吩嗪鎓甲酯（1-甲氧基吩嗪硫酸甲酯，1-methoxyPMS）是一种在烟酰胺腺嘌呤二核苷酸（磷酸）（NAD(P)H）和四唑盐染料之间具有光化学稳定性的电子介质。我们已经检测了1-甲氧基吩嗪硫酸甲酯作为电子介质在人乳酸脱氢酶（LDH，EC 1.1.1.27）检测以及电泳后LDH同工酶活性染色中的效率。1-甲氧基吩嗪硫酸甲酯作为NADH和硝基四氮唑蓝之间的电子介质，其转换数为10 - 12 s⁻¹，这一数值与吩嗪硫酸甲酯（PMS）的转换数相同。用1-甲氧基吩嗪硫酸甲酯和用黄递酶测定的人血清中估计的LDH活性之间的相关系数为0.998。在人血清LDH同工酶的活性染色中，1-甲氧基吩嗪硫酸甲酯成功替代了不稳定的PMS，背景染色较少。我们得出结论，1-甲氧基吩嗪硫酸甲酯将有助于诊断重要性酶的活性检测和活性染色的常规方法。