Hauswirth W W, Laipis P J, Gilman M E, O'Brien T W, Michaels G S, Rayfield M A
Gene. 1980 Jan;8(2):193-209. doi: 10.1016/0378-1119(80)90037-2.
Using a physical map of bovine mitochondrial DNA derived from the liver of a single Holstein cow, we have determined the location of the genes specifying the large and small ribosomal RNAs by hybridization analysis and electron microscopic observations of R-loop forms. Also, the position of the origin of DNA replication (D-loop) has been located by electron microscopy. Additionally, the direction of D-loop expansion and the polarity of the large and small ribosomal RNA genes were determined.
利用从一头荷斯坦奶牛肝脏中提取的牛线粒体DNA物理图谱，我们通过杂交分析以及对R环形式的电子显微镜观察，确定了指定大、小核糖体RNA的基因位置。此外，DNA复制起点（D环）的位置已通过电子显微镜定位。另外，还确定了D环扩展的方向以及大、小核糖体RNA基因的极性。
