粗糙脉孢菌线粒体DNA上的核糖体RNA基因是相邻的。

The ribosomal RNA genes on Neurospora crassa mitochondrial DNA are adjacent.

作者信息

Terpstra P, Holtrop M, Kroon A M

出版信息

Biochim Biophys Acta. 1977 Sep 20;478(2):146-55. doi: 10.1016/0005-2787(77)90178-2.

Abstract

Hybridization of separated 24 S and 17 S ribosomal RNA from Neurospora crassa mitochondrial ribosomes to restriction fragments of mitochondrial DNA leads to the conclusion that the large and small ribosomal RNA are adjacent on the restriction endonuclease cleavage map of the DNA. The distance between the two genes is estimated at 900 basepairs. This result is consistent with the existence of a ribosomal precursor RNA in N. crassa mitochondria and is in contrast to the situation in yeast, where the ribosomal genes are far apart on the mitochondrial DNA. The position of the ribosomal RNA genes on the cleavage map of N. crassa mtDNA provides a start for ordering the Hind III restriction fragments.

摘要

将粗糙脉孢菌线粒体核糖体中分离出的24 S和17 S核糖体RNA与线粒体DNA的限制性片段进行杂交，得出如下结论：在DNA的限制性内切酶切割图谱上，大、小核糖体RNA是相邻的。这两个基因之间的距离估计为900个碱基对。这一结果与粗糙脉孢菌线粒体中存在核糖体前体RNA的情况相符，与酵母的情况相反，酵母线粒体DNA上的核糖体基因相距甚远。粗糙脉孢菌线粒体DNA切割图谱上核糖体RNA基因的位置为对Hind III限制性片段进行排序提供了起点。