Prakobphol A, Linzer R, Genco R J
Infect Immun. 1980 Jan;27(1):150-7. doi: 10.1128/iai.27.1.150-157.1980.
A rhamnose-containing polysaccharide (RCP) was extracted and purified from cell walls of Streptococcus mutans B13 (serotype d) and was chemically and immunologically characterized. Walls were initially extracted with 5% trichloroacetic acid at 4 degrees C to remove the serotype antigen and were then sequentially extracted with increasing concentrations of hot acid. Extracts lacking galactose were combined and chromatographed on a column of diethylaminoethyl--Sephadex A25. The purified RCP contained 90% carbohydrate, 1.4% protein, and 0.16% phosphorus. Analysis by gas chromatography indicated that the RCP was composed of rhamnose and glucose in a 1.6:1 ratio. RCP was immunogenic in rabbits when animals were immunized with whole cells or cell walls. Antisera prepared against partially extracted cell walls of B13 appeared specific for RCP. These sera were not reactive with purified serotype d antigen or lipoteichoic acid in passive hemagglutination assays or by agar gel diffusion. The RCP appeared to be a cell wall polysaccharide that was both chemically and immunologically distinct from the serotype d antigen.
从变形链球菌B13（血清型d）细胞壁中提取并纯化了一种含鼠李糖的多糖（RCP），并对其进行了化学和免疫学特性分析。细胞壁最初在4℃下用5%三氯乙酸提取以去除血清型抗原，然后依次用浓度递增的热酸提取。将不含半乳糖的提取物合并，并在二乙氨基乙基-葡聚糖A25柱上进行色谱分析。纯化的RCP含有90%的碳水化合物、1.4%的蛋白质和0.16%的磷。气相色谱分析表明，RCP由鼠李糖和葡萄糖以1.6:1的比例组成。当用全细胞或细胞壁免疫兔子时，RCP具有免疫原性。针对B13部分提取的细胞壁制备的抗血清似乎对RCP具有特异性。在被动血凝试验或琼脂凝胶扩散试验中，这些血清与纯化的血清型d抗原或脂磷壁酸无反应。RCP似乎是一种细胞壁多糖，在化学和免疫学上均与血清型d抗原不同。
