Okahashi N, Nishida Y, Koga T, Hamada S
Microbiol Immunol. 1984;28(4):407-13. doi: 10.1111/j.1348-0421.1984.tb00692.x.
Serotype h carbohydrate antigen was prepared from cell walls of Streptococcus mutans strain MFe28 of monkey origin. The h antigen was extracted from the cell walls with 5% trichloracetic acid at 4 C, and purified by DEAE-Sephadex A-25 ion exchange chromatography followed by Sephacryl S-300 gel filtration. The purified antigen was composed of galactose (75%), glucose (16%), and rhamnose (3%). Although the antiserum against whole cells of S. mutans MFe28 gave a strong cross reaction with serotype d S. mutans, serotype h-specific antiserum could be obtained by adequate adsorption. The precipitin reactions and hapten inhibition test using serotype h-specific antiserum showed that galactose, glucose, and their derivative sugars were markedly potent inhibitors. It was concluded that the serotype h antigen is immunologically distinguishable from the known serotypes of S. mutans, although it is closely related to serotype d antigen of S. mutans.
血清型h碳水化合物抗原是从源自猴子的变形链球菌MFe28菌株的细胞壁制备的。h抗原在4℃下用5%三氯乙酸从细胞壁中提取，并通过DEAE-葡聚糖A-25离子交换色谱，随后进行Sephacryl S-300凝胶过滤进行纯化。纯化的抗原由半乳糖（75%）、葡萄糖（16%）和鼠李糖（3%）组成。尽管抗变形链球菌MFe28全细胞的抗血清与血清型d变形链球菌有强烈的交叉反应，但通过适当吸附可获得血清型h特异性抗血清。使用血清型h特异性抗血清的沉淀反应和半抗原抑制试验表明，半乳糖、葡萄糖及其衍生糖是明显有效的抑制剂。得出的结论是，血清型h抗原在免疫上与变形链球菌的已知血清型不同，尽管它与变形链球菌的血清型d抗原密切相关。
