培养条件对变形链球菌三甲基硅烷基糖谱的影响。
Effect of cultural conditions on trimethylsilyl-sugar profiles of Streptococcus mutans.
作者信息
Drucker D B, Aluyi H S
出版信息
Infect Immun. 1980 Jan;27(1):32-7. doi: 10.1128/iai.27.1.32-37.1980.
The cellular carbohydrates of Streptococcus mutans NCTC 10832 were converted to methyl glycoside trimethylsilyl ethers and analyzed by gas-liquid chromatography. The resulting profiles revealed the presence of glycerol, xylose, rhamnose, galactose, glucose, N-acetylglucosamine, and N-acetylmuramic acid. The proportions of monosaccharides in the profile were found to be stable with respect to changing growth temperature or duration of growth. However, the type of medium used, and its carbohydrate content, did significantly alter the profile, whereas gaseous atmosphere exerted a lesser effect on carbohydrate composition. By controlling growth parameters, we obtained reproducible profiles. Whole cells are probably a satisfactory alternative to highly purified cell walls.
变形链球菌NCTC 10832的细胞碳水化合物被转化为甲基糖苷三甲基硅醚，并通过气液色谱法进行分析。所得图谱显示存在甘油、木糖、鼠李糖、半乳糖、葡萄糖、N-乙酰葡糖胺和N-乙酰胞壁酸。发现图谱中单糖的比例在生长温度或生长持续时间变化时是稳定的。然而，所用培养基的类型及其碳水化合物含量确实会显著改变图谱，而气态气氛对碳水化合物组成的影响较小。通过控制生长参数，我们获得了可重复的图谱。全细胞可能是高度纯化细胞壁的令人满意的替代品。
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