MACLEOD P, BROWN J P
J Bacteriol. 1963 May;85(5):1056-60. doi: 10.1128/jb.85.5.1056-1060.1963.
MacLeod, Patricia (University of Connecticut, Storrs) and Joseph P. Brown. Fatty acid composition of lipids from Streptococcus cremoris and Streptococcus lactis var. maltigenes. J. Bacteriol. 85:1056-1060. 1963.-A method for the extraction of lipids from bacterial cells, involving grinding in a ball mill, is described. Cells of Streptococcus lactis var. maltigenes and S. cremoris yielded 5.3 and 5.5%, respectively, of their dry cellular weights as lipid. The lipids of both organisms were separated into three fractions by thin-layer chromatography. Two of these fractions have been classified: one fraction containing phospho- and perhaps glycolipids and the other containing triglycerides. The unidentified fraction was probably either mono- or diglyceride in nature. The fatty acid composition of the total lipid as well as that of each of the separated lipid components from the two lactic strains were compared.
麦克劳德,帕特里夏(康涅狄格大学斯托尔斯分校)和约瑟夫·P·布朗。嗜热链球菌和产麦芽糖乳链球菌脂质的脂肪酸组成。《细菌学杂志》85:1056 - 1060。1963年。——描述了一种从细菌细胞中提取脂质的方法,该方法包括在球磨机中研磨。产麦芽糖乳链球菌和嗜热链球菌的细胞分别产生了其干细胞重量5.3%和5.5%的脂质。两种微生物的脂质通过薄层色谱法分离成三个部分。其中两个部分已被分类:一个部分含有磷酸脂和可能的糖脂,另一个部分含有甘油三酯。未鉴定的部分在性质上可能是单甘油酯或二甘油酯。比较了两种乳酸菌株总脂质以及每个分离脂质成分的脂肪酸组成。