Weimar V L, Squires E L, Knox R J
Invest Ophthalmol Vis Sci. 1980 Apr;19(4):350-61.
Freeze-thaw injuries of rabbit corneas were treated with mesodermal growth factor (MGF) in organ culture and the repair of the endothelium followed by light microscopic examination of endothelial whole mounts. MGF-treated corneas showed an increased number of mitotic figures. In addition, MGF accelerated the rate of cell hypertrophy, formation of spindle-shaped cells, and the rate of their migration into the killed zone so that Descemet's was covered more rapidly than was that of the controls. In MGF-treated corneas, the cells lost their fibroblast-like appearance and became endothelial-like in appearance sooner than did the untreated corneas. A short period of treatment (6 hr) was sufficient to stimulate the repair processes described above. The healing responses obtained in organ culture for control eyes approximately those described in the literature for freeze-thaw injuries to the rabbit endothelium in vivo. The data presented also show that it is possible to accelerate endothelial regeneration in wound healing.
在器官培养中，用中胚层生长因子（MGF）处理兔角膜的冻融损伤，并通过对内皮全片进行光学显微镜检查来观察内皮的修复情况。经MGF处理的角膜显示有丝分裂象数量增加。此外，MGF加快了细胞肥大的速度、纺锤形细胞的形成以及它们向内皮损伤区迁移的速度，因此，与对照组相比，MGF处理组角膜后弹力层被覆盖的速度更快。在经MGF处理的角膜中，细胞比未处理的角膜更快地失去成纤维细胞样外观，而呈现出内皮细胞样外观。短时间（6小时）的处理足以刺激上述修复过程。在器官培养中，对照眼所获得的愈合反应与文献中描述的兔内皮细胞在体内发生冻融损伤后的愈合反应大致相同。所呈现的数据还表明，在伤口愈合过程中加速内皮细胞再生是可能的。
