de Boccardo G, Drayer D, Rubin A L, Novogrodsky A, Reidenberg M M, Stenzel K H
Clin Exp Immunol. 1985 Jan;59(1):69-76.
The present study examined the effect of two drugs, which contain either an aromatic amine or hydrazine moiety and are known to induce lupus like syndromes in man (procainamide and hydralazine) and an aliphatic amine (dansylcadaverine), on pokeweed mitogen (PWM)-induced B cell production of immunoglobulin G (IgG). These compounds all inhibited IgG production and generation of IgG plaque forming cells, whereas derivatives of them, without free amine groups, had little or no effect. The compounds inhibited differentiation of B cells to plasma cells, rather than production and secretion of IgG. Mitogen free culture supernatants of peripheral blood mononuclear cells (PBM) activated by the oxidizing mitogen, neuraminidase and galactose oxidase (NAGO), prevented the inhibition of B cell maturation. Moreover, incubation of NAGO treated PBM with hydralazine prevented the production of soluble factors capable of promoting B cell maturation in the presence of hydralazine. We conclude from these studies that procainamide, hydralazine and dansylcadaverine inhibit PWM-induced B cell maturation to plasma cells by an indirect mechanism, via inhibition of production of lymphokines by helper cells. The primary amine or hydrazine group appears to be required for the inhibitory effect, since analogues of the inhibitory compounds, without primary amine groups, are non-inhibitory.
本研究检测了两种药物对美洲商陆丝裂原（PWM）诱导的B细胞产生免疫球蛋白G（IgG）的影响。这两种药物一种含有芳香胺或肼基部分，已知可在人体内诱发狼疮样综合征（普鲁卡因胺和肼屈嗪），另一种是脂肪族胺（丹磺酰尸胺）。这些化合物均抑制IgG的产生以及IgG噬斑形成细胞的生成，而它们没有游离胺基的衍生物则几乎没有影响或没有影响。这些化合物抑制B细胞向浆细胞的分化，而不是IgG的产生和分泌。由氧化型丝裂原神经氨酸酶和半乳糖氧化酶（NAGO）激活的外周血单个核细胞（PBM）的无丝裂原培养上清液可防止B细胞成熟受到抑制。此外，用肼屈嗪处理NAGO处理过的PBM，可防止在有肼屈嗪存在的情况下产生能够促进B细胞成熟的可溶性因子。我们从这些研究中得出结论，普鲁卡因胺、肼屈嗪和丹磺酰尸胺通过间接机制，即通过抑制辅助细胞产生淋巴因子，抑制PWM诱导的B细胞成熟为浆细胞。由于没有伯胺基的抑制性化合物类似物无抑制作用，因此抑制作用似乎需要伯胺或肼基。
