聚合物低温保护剂在生物超微结构保存中的应用。II. 生理效应。

Polymeric cryoprotectants in the preservation of biological ultrastructure. II. Physiological effects.

作者信息

Echlin P, Skaer H B, Gardiner B O, Franks F, Asquith M H

出版信息

J Microsc. 1977 Aug;110(3):239-55. doi: 10.1111/j.1365-2818.1977.tb00035.x.

DOI:10.1111/j.1365-2818.1977.tb00035.x

Abstract

A study has been made of the physiological effects of three non-penetrating polymeric cryoprotective agents on sixteen different plant and animal cells and tissues. The cryoprotectants, when used at concentrations at which they are effective in preventing ice-crystal formation, generally have a lower toxicity to cells and tissue than similar concentrations of glycerol. The relatively low toxicity of these substances suggests that they would be more suitable as cryoprotectants for morphological and analytical studies than the commonly used low molecular weight compounds.

摘要

一项针对三种非渗透性聚合物冷冻保护剂对16种不同动植物细胞及组织的生理效应进行了研究。这些冷冻保护剂在有效防止冰晶形成的浓度下使用时，通常对细胞和组织的毒性低于相同浓度的甘油。这些物质相对较低的毒性表明，与常用的低分子量化合物相比，它们作为形态学和分析研究的冷冻保护剂更为合适。