聚合物低温保护剂在生物超微结构保存中的应用。III. 形态学方面

Polymeric cryoprotectants in the preservation of biological ultrastructure. III. Morphological aspects.

作者信息

Skaer H B, Franks F, Asquith M H, Echlin P

出版信息

J Microsc. 1977 Aug;110(3):257-70. doi: 10.1111/j.1365-2818.1977.tb00036.x.

DOI:10.1111/j.1365-2818.1977.tb00036.x

Abstract

Two high molecular weight polymers, polyvinylpyrrolidone (PVP) and hydroxyethyl starch (HES), have been used as cryoprotectants for preparing specimens to be freeze fractured. Solutions of 25% (w/w) suppress the formation of intracellular ice in single cells and tissue blocks from both plants and animals to the extent that fine structural details of the cell can be elucidates. The mode of action of these cryoprotectants, together with the structures they reveal and the peculiar advantages attached to their use, is discussed.

摘要

两种高分子量聚合物，聚乙烯吡咯烷酮（PVP）和羟乙基淀粉（HES），已被用作冷冻保护剂来制备用于冷冻断裂的标本。25%（w/w）的溶液能抑制动植物单细胞和组织块中细胞内冰的形成，从而能够阐明细胞的精细结构细节。本文讨论了这些冷冻保护剂的作用方式，以及它们所揭示的结构和使用它们所具有的独特优势。