Ijiri K, Egami N
Mutat Res. 1980 Feb;69(2):241-8. doi: 10.1016/0027-5107(80)90089-5.
When sperm of the fish Oryzias latipes were irradiated with ultraviolet light and allowed to fertilize normal eggs, the so-called "Hertwig effect" was observed, with a dose-dependent decrease in survival rate at low doses (0-27 J . m-2) but a better survival rate at higher dose ranges. Illumination with visible light after fertilization (10-70 min after insemination) showed the existence of photoreactivation (PR), demonstrating that pyrimidine dimers are a lesion in sperm DNA that is mainly responsible for the UV-caused Hertwig effect. Genetic analysis, in which sperm from a wild-type of this fish was used, showed that, after UV-irradiation at the high dose range, male nuclei did not participate in embryonic development (a gynogenetic haploid condition). Embryos having only a maternal set of chromosomes could develop no further than stage 27. Only the visible light during the early part (until around 20-30 min after insemination, at 25 degrees C) of the single-cell stage was effective for PR; illumination thereafter was not.
当用紫外线照射青鳉鱼的精子并使其使正常卵子受精时，观察到了所谓的“赫特维希效应”，在低剂量（0 - 27 J·m⁻²）下存活率呈剂量依赖性下降，但在较高剂量范围内存活率更高。受精后（授精后10 - 70分钟）用可见光照射显示存在光复活作用（PR），这表明嘧啶二聚体是精子DNA中的一种损伤，主要导致紫外线引起的赫特维希效应。使用这种鱼野生型的精子进行的遗传分析表明，在高剂量范围的紫外线照射后，雄核不参与胚胎发育（雌核发育单倍体状态）。仅具有母本染色体组的胚胎发育到27阶段后就无法继续发育。仅单细胞阶段早期（在25℃下授精后约20 - 30分钟左右）的可见光对光复活有效；此后的照射则无效。
