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斑马鱼眼睛的三维布里渊显微镜数据集。

A 3D Brillouin microscopy dataset of the zebrafish eye.

作者信息

Sánchez-Iranzo Héctor, Bevilacqua Carlo, Diz-Muñoz Alba, Prevedel Robert

机构信息

Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

出版信息

Data Brief. 2020 Mar 17;30:105427. doi: 10.1016/j.dib.2020.105427. eCollection 2020 Jun.

DOI:10.1016/j.dib.2020.105427
PMID:32274409
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7132165/
Abstract

In this work we present three-dimensional (3D) measurements of Brillouin scattering spectra of the zebrafish larvae eye. This dataset was obtained by Brillouin microscopy, an emerging all-optical and non-contact technique that gives access to material properties through the process of Brillouin scattering. Herein, we share a representative 3D dataset of spectral properties of 48-52 h post-fertilization (hpf) zebrafish embryos. These spectral properties can be related to a complex longitudinal modulus and thus elastic and viscous properties given knowledge of refractive index and material density. The dataset encompasses the crystalline lens as well as several different retinal layers. This data provides a valuable resource as well as a starting point for researchers interested in the mechanobiology of vertebrate eye development.

摘要

在这项工作中,我们展示了斑马鱼幼体眼睛的布里渊散射光谱的三维(3D)测量结果。该数据集是通过布里渊显微镜获得的,这是一种新兴的全光学非接触技术,可通过布里渊散射过程获取材料特性。在此,我们分享了受精后48 - 52小时(hpf)斑马鱼胚胎光谱特性的代表性3D数据集。已知折射率和材料密度的情况下,这些光谱特性可与复杂的纵向模量相关,进而与弹性和粘性特性相关。该数据集涵盖晶状体以及几个不同的视网膜层。这些数据为对脊椎动物眼睛发育的力学生物学感兴趣的研究人员提供了宝贵的资源和起点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3097/7132165/3febe8570397/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3097/7132165/b797e15171f2/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3097/7132165/3febe8570397/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3097/7132165/b797e15171f2/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3097/7132165/3febe8570397/gr2.jpg

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本文引用的文献

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Brillouin microscopy: an emerging tool for mechanobiology.布里渊显微镜:力学生物学的新兴工具。
Nat Methods. 2019 Oct;16(10):969-977. doi: 10.1038/s41592-019-0543-3. Epub 2019 Sep 23.
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Imaging mechanical properties of sub-micron ECM in live zebrafish using Brillouin microscopy.使用布里渊显微镜成像活斑马鱼中亚微米细胞外基质的力学特性。
Biomed Opt Express. 2019 Feb 25;10(3):1420-1431. doi: 10.1364/BOE.10.001420. eCollection 2019 Mar 1.
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Coordinated Morphogenetic Mechanisms Shape the Vertebrate Eye.协调的形态发生机制塑造脊椎动物眼睛。
Elife. 2024 Jan 12;13:e76421. doi: 10.7554/eLife.76421.
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Amoeboid-like migration ensures correct horizontal cell layer formation in the developing vertebrate retina.阿米巴样迁移确保了脊椎动物发育过程中水平细胞层的正确形成。
Elife. 2022 May 31;11:e76408. doi: 10.7554/eLife.76408.
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Mapping Tumor Spheroid Mechanics in Dependence of 3D Microenvironment Stiffness and Degradability by Brillouin Microscopy.通过布里渊显微镜映射肿瘤球体力学与三维微环境硬度和可降解性的关系
Cancers (Basel). 2021 Nov 5;13(21):5549. doi: 10.3390/cancers13215549.
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High-resolution microscopy and spectroscopy datasets meet .高分辨率显微镜和光谱数据集相遇。
Data Brief. 2020 Apr 21;30:105596. doi: 10.1016/j.dib.2020.105596. eCollection 2020 Jun.
Front Neurosci. 2017 Dec 20;11:721. doi: 10.3389/fnins.2017.00721. eCollection 2017.
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Multistage VIPA etalons for high-extinction parallel Brillouin spectroscopy.用于高消光比并行布里渊光谱的多级VIPA标准具
Opt Express. 2011 May 23;19(11):10913-22. doi: 10.1364/OE.19.010913.
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