Namkung P C, Moe R E, Petra P H
Cancer Res. 1979 Mar;39(3):1124-5.
The effect of freezing on the stability of the estrogen receptors in human breast tumors was measured. Portions of three tumors kept frozen for 3 days after removal from the patients were analyzed by the sucrose density gradient method. The initial measurements demonstrated positive receptor values. The remaining portions were kept frozen for 30 days, and the receptor values were reevaluated. The 8S receptor was 80% destroyed under these freezing conditions. The 4S receptor was found to be more stable. The inactivation reflects a loss of specific binding since the protein concentration was similar in the analyses. These results strongly indicate that human breast tumor specimens should be analyzed as soon as possible or within 3 weeks after freezing.
测定了冷冻对人乳腺肿瘤中雌激素受体稳定性的影响。从患者身上切除后冷冻保存3天的三个肿瘤样本，采用蔗糖密度梯度法进行分析。初始测量显示受体值为阳性。其余样本冷冻保存30天，然后重新评估受体值。在这些冷冻条件下，8S受体有80%被破坏。发现4S受体更稳定。由于分析中蛋白质浓度相似，这种失活反映了特异性结合的丧失。这些结果强烈表明，人乳腺肿瘤标本应尽快分析，或在冷冻后3周内进行分析。
