Coppola A, Viggiani E, Salzarulo L, Rasile G
Am J Pathol. 1980 Apr;99(1):175-92.
Hematoporphyrin, when activated by light, produces lethal effects on lymphoma cells in vivo and in vitro. Mice bearing subcutaneous lymphomatous nodules received hematoporphyrin and two days later were exposed to light. Extensive necrosis occurred within 48 hours after exposure to light. Since no evidence has yet been presented as to what part of the cell is first affected by this treatment, ultrastructural studies were undertaken using an in vitro system. The first morphologic change in leukemic cells occurred within 5 minutes within the mitochondria. First they appeared markedly contracted and subsequently swollen, and ultimately they became completely disrupted. It is hypothesized that possibly mitochondria have a greater affinity for the hematoporphyrin and therefore become the first organelles to be affected.
血卟啉在光激活后，可在体内和体外对淋巴瘤细胞产生致死效应。携带皮下淋巴瘤结节的小鼠接受血卟啉注射，两天后暴露于光线下。光照后48小时内出现广泛坏死。由于尚未有证据表明细胞的哪一部分首先受到这种治疗的影响，因此使用体外系统进行了超微结构研究。白血病细胞的第一个形态学变化发生在线粒体内5分钟内。首先它们明显收缩，随后肿胀，最终完全破裂。据推测，线粒体可能对血卟啉具有更大的亲和力，因此成为第一个受到影响的细胞器。
