Thompson D L, Elgert K D, Gross W B, Siegel P B
Am J Vet Res. 1980 Jan;41(1):91-6.
Lines of chickens genetically selected for high (HPC)-and low-plasma corticosterone (LPC) titers were assessed for cell-mediated immunity during Marek's disease tumorigenesis. Lymphocyte transformation (using phytohemagglutinin and 2,4-dinitrofluorobenzene-bovine serum complex) and mitogen-induced cellular cytotoxicity were evaluated with peripheral blood leukocytes. Chickens from the LPC line showed a greater lymphocyte transformation response than those from the HPC line. All tumor-bearing chickens demonstrated a markedly suppressed blastogenic response. Although the blastogenic response was suppressed in those chickens infected with Marek's disease virus but not having gross tumors when compared with the steroid level, this response was greater than that of chickens with tumors and less than that of normal controls. In comparison with LPC-line chickens, HPC-line chickens demonstrated a decreased mitogen-induced cellular cytotoxicity. A decreased mitogen-induced cellular cytotoxicity was observed in both steroid lines concomitant with the onset and severity of tumors. The impaired cell-mediated response observed in the HPC line was associated with an increased tumor incidence and a greater mortality than that observed in the LPC line. Metyrapone, a chemical which blocks adrenal corticosterone synthesis, transiently enhanced mitogen-induced cellular cytotoxicity and initiated tumor regression in chickens debilitated with Marek's disease tumors.
对经过基因选择、具有高血浆皮质酮（HPC）和低血浆皮质酮（LPC）滴度的鸡群，在马立克氏病肿瘤发生过程中进行细胞介导免疫评估。使用外周血白细胞评估淋巴细胞转化（采用植物血凝素和2,4-二硝基氟苯-牛血清复合物）和丝裂原诱导的细胞毒性。LPC系的鸡比HPC系的鸡表现出更强的淋巴细胞转化反应。所有患肿瘤的鸡均表现出明显的增殖反应抑制。尽管与类固醇水平相比，感染马立克氏病病毒但无明显肿瘤的鸡的增殖反应受到抑制，但该反应比患肿瘤的鸡更强且比正常对照鸡更弱。与LPC系鸡相比，HPC系鸡表现出丝裂原诱导的细胞毒性降低。在两个类固醇系中，随着肿瘤的发生和严重程度，均观察到丝裂原诱导的细胞毒性降低。在HPC系中观察到的细胞介导反应受损与肿瘤发生率增加和死亡率高于LPC系有关。美替拉酮是一种阻断肾上腺皮质酮合成的化学物质，可短暂增强丝裂原诱导的细胞毒性，并使患有马立克氏病肿瘤的衰弱鸡的肿瘤消退。
