使用乙二醇稳定的液体NAD在GEMSAEC上测量三种酶。

Utilization of a glycol-stabilized liquid NAD for the measurement of three enzymes on the GEMSAEC.

作者信息

Carter P, Rose M E

出版信息

Clin Biochem. 1980 Feb;13(1):38-40. doi: 10.1016/s0009-9120(80)90542-1.

Abstract

Some analytical parameters have been investigated for a recently described stabilized liquid coenzyme technology in which water-free NAD is dissolved in 1,2 propanediol. Correlation for 108 specimens assayed for AST, ALT and LD with a reference method in which glycol-based NAD was absent was greater than or equal to 0.998 with near identical reproducibility over a period of at least 107 days. Mean recovery of exogenous serum enzymes in this linear kinetic assay is 103%. With the option of mixing only the volume of reagent needed for the enzymatic assay, waste can be eliminated as compared to more costly preparations stabilized by lyophilization. Hazards from an impure water supply are avoided since no reconstituting volume is required.

摘要

对于最近描述的一种稳定化液体辅酶技术，已对一些分析参数进行了研究，该技术是将无水烟酰胺腺嘌呤二核苷酸（NAD）溶解于1,2 - 丙二醇中。采用不含二醇基NAD的参考方法对108份标本进行天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）和乳酸脱氢酶（LD）检测时，相关性大于或等于0.998，且在至少107天的时间内具有几乎相同的重现性。在这种线性动力学检测中，外源血清酶的平均回收率为103%。由于只需混合酶促检测所需的试剂体积，与更昂贵的冻干稳定制剂相比，可以消除浪费。由于无需复溶体积，避免了来自不纯水源的危害。