普通脱硫弧菌提取物合成半胱氨酸
Cysteine synthesis by Desulfovibrio vulgaris extracts.
作者信息
Gevertz D, Amelunxen R, Akagi J M
出版信息
J Bacteriol. 1980 Mar;141(3):1460-2. doi: 10.1128/jb.141.3.1460-1462.1980.
Abstract
Extracts of Desulfovibrio vulgaris were found to contain serine transacetylase and cysteine synthase activities. When extracts were incubated with bisulfite and o-acetylserine, or acetyl coenzyme A plus L-serine, under a hydrogen atmosphere, cysteine was formed. Pyruvate served as a reductant for bisulfite reduction to sulfide and concomitantly provided the acetyl moiety for acetyl coenzyme A formation. Consequently, when extracts were incubated with pyruvate, bisulfite, and L-serine, cysteine synthesis resulted.
摘要
已发现普通脱硫弧菌提取物含有丝氨酸转乙酰酶和半胱氨酸合酶活性。当提取物在氢气氛围下与亚硫酸氢盐和O-乙酰丝氨酸，或乙酰辅酶A加L-丝氨酸一起孵育时，会形成半胱氨酸。丙酮酸作为亚硫酸氢盐还原为硫化物的还原剂，并同时为乙酰辅酶A的形成提供乙酰部分。因此，当提取物与丙酮酸、亚硫酸氢盐和L-丝氨酸一起孵育时，会产生半胱氨酸合成。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A modification of the nitroprusside method of analysis for glutathione.谷胱甘肽硝普盐分析法的一种改良方法。
Arch Biochem. 1951 Feb;30(2):217-25.
3
Electron carries for the phosphoroclastic reaction of Desulfovibrio desulfuricans.脱硫脱硫弧菌磷酸解酮酶反应的电子载体。
J Biol Chem. 1967 May 25;242(10):2478-83.
4
The enzymic synthesis of L-cysteine in Escherichia coli and Salmonella typhimurium.大肠杆菌和鼠伤寒沙门氏菌中L-半胱氨酸的酶促合成
J Biol Chem. 1966 Nov 10;241(21):4955-65.
5
Cysteine and S-sulphocysteine biosynthesis in bacteria.细菌中半胱氨酸和S-磺基半胱氨酸的生物合成
Arch Mikrobiol. 1971;77(2):165-84. doi: 10.1007/BF00408609.
6
The purification and characterization of O-acetylserine sulfhydrylase-A from Salmonella typhimurium.鼠伤寒沙门氏菌O-乙酰丝氨酸巯基酶-A的纯化与特性分析
J Biol Chem. 1969 May 10;244(9):2418-27.
7
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