Hellio R, Ryter A
J Cell Sci. 1980 Feb;41:89-104. doi: 10.1242/jcs.41.1.89.
The disappearance of Wheat Germ Agglutinin (WGA) receptors from the membrane of yeast-engulfing-phagocytic cups in Dictyostelium suggested that these receptors could play a role in yeast adsorption or ingestion. This problem was approached by comparing the fate of WGA, Concanavalin A (Con A) and cationized ferritin (CF) and their effects on the phagocytosis of yeast, bacteria and latex beads. It can be concluded that CF capped in about 30 min and inhibited phagocytosis of any kind of particles for about 15 min. Con A capped in 20--60 min and inhibited phagocytosis of all particles for 1 h 30 min. The time at which phagocytosis started to occur corresponded approximately to the moment at which large areas of plasma membrane were totally devoid of marker. WGA did not cap but induced the formation of large and tight aggregates. The surface of the peripheral cells progressively released WGA in 1 h 30 min. Afterwards, the cells were able to ingest latex beads and bacteria but did not phagocytoze yeast. The latter started to be adsorbed onto the cells and to be ingested only 1 h later. Double labelling experiments showed that CF and Con A receptors were still absent in the plasma membrane, when phagocytosis of any kind of particles started to occur. WGA-labelled cells ingested latex beads and bacteria when their plasma membrane was still devoid of WGA receptors but were able to ingest yeast only after their regeneration. These observations strongly suggest that WGA receptors may correspond to specific receptors for yeast phagocytosis.
盘基网柄菌中酵母吞噬杯膜上麦胚凝集素（WGA）受体的消失表明，这些受体可能在酵母吸附或摄取过程中发挥作用。通过比较WGA、伴刀豆球蛋白A（Con A）和阳离子化铁蛋白（CF）的命运及其对酵母、细菌和乳胶珠吞噬作用的影响来研究这个问题。可以得出结论，CF在约30分钟内形成帽状结构，并在约15分钟内抑制任何类型颗粒的吞噬作用。Con A在20 - 60分钟内形成帽状结构，并在1小时30分钟内抑制所有颗粒的吞噬作用。吞噬作用开始发生的时间大约与大片质膜完全没有标记物的时刻相对应。WGA没有形成帽状结构，但诱导形成大而紧密的聚集体。外周细胞表面在1小时30分钟内逐渐释放WGA。此后，细胞能够摄取乳胶珠和细菌，但不能吞噬酵母。酵母在1小时后才开始吸附到细胞上并被摄取。双重标记实验表明，当任何类型颗粒的吞噬作用开始发生时，质膜中仍然没有CF和Con A受体。WGA标记的细胞在其质膜仍然没有WGA受体时摄取乳胶珠和细菌，但只有在其再生后才能摄取酵母。这些观察结果强烈表明，WGA受体可能对应于酵母吞噬作用的特异性受体。
