Ryter A, Hellio R
J Cell Sci. 1980 Feb;41:75-88. doi: 10.1242/jcs.41.1.75.
The study of plasma membrane and phagosome membrane of Dictyostelium discoideum was performed using 2 lectins: concanavalin A (Con A) and Wheat Germ Agglutinin (WGA), and 2 markers of anionic sites: colloidal iron hydroxide (CIH) and cationized ferritin (CF). These labellings were applied to fixed partially broken cells, which had ingested a large quantity of yeast. They showed that Con A and CF labelled both the outer and inner faces of the plasma membrane, whereas CIH and WGA were deposited on the outer face only. Phagosome membranes displayed the same location as the plasma membrane for Con A, CF and CIH even in very old phagosomes. This suggests that most receptors of these 3 markers were not degraded by hydrolases. In contrast, phagosome membranes of young and old phagosomes did not react with WGA. When labellings were made on yeast phagocytozing cells, the membrane of phagocytic cups were also devoid of WGA, while it was labelled with the 3 other markers. The absence of WGA labelling was not observed during ingestion of bacteria and latex beads, suggesting a specific relationship existed between yeast cells and WGA receptors.
伴刀豆球蛋白A（Con A）和麦胚凝集素（WGA），以及两种阴离子位点标记物：氢氧化胶体铁（CIH）和阳离子铁蛋白（CF），对盘基网柄菌的质膜和吞噬体膜进行了研究。这些标记物被应用于固定的部分破碎细胞，这些细胞摄取了大量酵母。结果表明，Con A和CF标记了质膜的外表面和内表面，而CIH和WGA仅沉积在外表面。即使在非常陈旧的吞噬体中，吞噬体膜对于Con A、CF和CIH的定位也与质膜相同。这表明这三种标记物的大多数受体未被水解酶降解。相反，年轻和陈旧吞噬体的吞噬体膜不与WGA发生反应。当对吞噬酵母的细胞进行标记时，吞噬杯的膜也没有WGA标记，而用其他三种标记物进行标记时则有。在摄取细菌和乳胶珠的过程中未观察到WGA标记的缺失，这表明酵母细胞与WGA受体之间存在特定关系。
