用于定量测定血浆、尿液和心肌组织中溴苄铵的灵敏气相色谱分析法。
Sensitive gas chromatographic assay for the quantitation of bretylium in plasma, urine and myocardial tissue.
作者信息
Patterson E, Stetson P, Lucchesi B R
出版信息
J Chromatogr. 1980 Jan 11;181(1):33-9. doi: 10.1016/s0378-4347(00)81266-9.
A sensitive analytical method has been developed for the quantitation of bretylium in plasma, urine and myocardial tissue. Bretylium and the internal standard, UM-360 (o-iodobenzyltrimethylammonium), are extracted and isolated as the iodide salts. Sodium benzenethiolate is added and the mixture heated to 100 degrees for one hour. This results in the formation of 2-bromobenzyl phenyl thioether and 2-iodobenzyl phenyl thioether, which can be separated and quantitated by gas chromatography. Good reliability and reproducibility can be obtained using electron-capture detection with quantities of bretylium as small as 1 ng.
已开发出一种灵敏的分析方法，用于定量测定血浆、尿液和心肌组织中的溴苄铵。溴苄铵和内标物UM - 360（邻碘苄基三甲基铵）以碘化物盐的形式被萃取和分离。加入苯硫酚钠并将混合物加热至100度持续一小时。这会导致形成2 - 溴苄基苯基硫醚和2 - 碘苄基苯基硫醚，它们可通过气相色谱法进行分离和定量。使用电子捕获检测法，即使溴苄铵的量低至1纳克，也能获得良好的可靠性和重现性。
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