Zia H, Proveaux W J, O'Donnell J P, Ma J K
J Chromatogr. 1980 Jan 11;181(1):77-84. doi: 10.1016/s0378-4347(00)81271-2.
A simple and accurate assay for the determination of griseofulvin and its metabolites in biological fluids using high-performance liquid chromatography is described. Using a reversed phase column and a mobile phase solvent of 45% acetonitrile in 0.1 M acetic acid, baseline separation of griseofulvin and several analogues was obtained. The described method allows one to quantitatively determine griseofulvin, 6-demethylgriseofulvin, and griseofulvic acid, a newly identified metabolite in man, in urine and plasma samples. Treatment of plasma samples prior to the analysis is simply made by deproteinizing the samples with an equal volume of acetonitrile. For urine samples, the procedure involves diethyl ether extraction with subsequent evaporation to dryness and reconstitution with the mobile phase solvent.
本文描述了一种使用高效液相色谱法测定生物流体中灰黄霉素及其代谢物的简单而准确的分析方法。使用反相柱和含45%乙腈的0.1M乙酸作为流动相溶剂，实现了灰黄霉素及其几种类似物的基线分离。所描述的方法能够定量测定尿液和血浆样本中的灰黄霉素、6-去甲基灰黄霉素以及人体中新鉴定出的代谢物灰黄霉素酸。血浆样本在分析前的处理只需用等体积的乙腈使样本脱蛋白。对于尿液样本，该程序包括用乙醚萃取，随后蒸发至干并用流动相溶剂复溶。
