Papp E, Magyar K, Schwarz H J
J Pharm Sci. 1976 Mar;65(3):441-3. doi: 10.1002/jps.2600650335.
A specific and quantitative liquid-solid chromatographic method for the determination of 6-demethylgriseofulvin in human urine is reported. The method consists of extraction into an organic solvent, addition of internal standard, and analysis by liquid-solid chromatography using a UV detector. Griseofulvin, if present, can be determined simultaneously. The sensitivity of the method is 6 mug/ml of urine. Total 6-demethylgrisefulvin is determined after hydrolysis of the glucuronide conjugate with glucuronidase-sulfatase enzyme solution. The method is well suited for the analysis of a large number of samples.
本文报道了一种用于测定人尿中6-去甲基灰黄霉素的特异性定量液固色谱法。该方法包括用有机溶剂萃取、加入内标，并用紫外检测器通过液固色谱进行分析。如有灰黄霉素存在，也可同时测定。该方法的灵敏度为6微克/毫升尿液。用葡糖醛酸糖苷酶-硫酸酯酶溶液水解葡糖醛酸缀合物后，测定总6-去甲基灰黄霉素。该方法非常适合分析大量样品。
