Hruby D E, Lynn D L, Kates J R
J Gen Virol. 1980 Apr;47(2):293-9. doi: 10.1099/0022-1317-47-2-293.
A new protein has been detected in the nuclei of vaccinia virus-infected cells. This protein has an apparent mol. wt. of 28000 (VP28) on SDS--polyacrylamide gels and has been detected in Triton X-100-treated nuclei of infected BSC-40, L-929 and CVC cells. Within the infected cells, VP 28 was synthesized maximally at 1 to 2 h p.i. in the cytoplasm and accumulated in the nuclei at 4 to 5 h p.i. The appearance of VP28 was not affected by cytosine arabinoside (25 microgram/ml), an inhibitor of virus DNA synthesis, or rifampicin (100 microgram/ml), an inhibitor of vaccinia assembly, but was inhibited by irradiation of the infecting virions; thus classifying it as an early vaccinia virus gene product. Nuclear--cytoplasmic mixing experiments suggested that the nuclear location of VP28 was not an artefact of the cell fractionation techniques employed. VP28 did not appear to be phosphorylated.
在感染痘苗病毒的细胞的细胞核中检测到一种新蛋白质。这种蛋白质在SDS-聚丙烯酰胺凝胶上的表观分子量为28000（VP28），并且已在经Triton X-100处理的受感染BSC-40、L-929和CVC细胞的细胞核中检测到。在受感染细胞内，VP28在感染后1至2小时在细胞质中最大量合成，并在感染后4至5小时在细胞核中积累。VP28的出现不受病毒DNA合成抑制剂阿糖胞苷（25微克/毫升）或痘苗组装抑制剂利福平（100微克/毫升）的影响，但受到感染性病毒粒子照射的抑制；因此将其归类为早期痘苗病毒基因产物。核质混合实验表明，VP28的核定位不是所采用的细胞分级分离技术的假象。VP28似乎未被磷酸化。
