Archard L C
J Gen Virol. 1983 Dec;64 ( Pt 12):2561-75. doi: 10.1099/0022-1317-64-12-2561.
It is well established that vaccinia virus infection induces the synthesis of virus-specific DNA in cytoplasmic 'factories', which are sites of virus-specific transcription. The present study demonstrates that vaccinia virus-specific DNA is synthesized also in the nuclei of infected cells with a similar time course. Direct observation and radiolabelling confirm the integrity of isolated nuclei. Reconstitution experiments and inhibitor studies demonstrate that virus-induced DNA is synthesized de novo within nuclei and does not result from cytoplasmic contamination. Cell-specific DNA synthesis is inhibited completely after infection and nuclei of infected cells then synthesize DNA which co-sediments with virus genomic DNA in denaturing gradients. Restriction endonuclease cleavage and hybridization with a virus-specific probe indicate that this is full-length, virus genomic DNA. The biological implications of this are discussed.
痘苗病毒感染可诱导在细胞质“工厂”中合成病毒特异性DNA，这些“工厂”是病毒特异性转录的场所，这一点已得到充分证实。本研究表明，痘苗病毒特异性DNA也在受感染细胞的细胞核中以类似的时间进程合成。直接观察和放射性标记证实了分离细胞核的完整性。重组实验和抑制剂研究表明，病毒诱导的DNA是在细胞核内从头合成的，并非细胞质污染所致。感染后细胞特异性DNA合成被完全抑制，然后受感染细胞的细胞核合成在变性梯度中与病毒基因组DNA共沉降的DNA。限制性内切酶切割和与病毒特异性探针杂交表明，这是全长的病毒基因组DNA。文中讨论了这一现象的生物学意义。
