Brackett K A, Townsend S F
J Morphol. 1980 Feb;163(2):203-15. doi: 10.1002/jmor.1051630208.
As part of a study of ulcer formation and healing, regeneration of colonic mucosa in rats was studied following placement of a surgical lesion. Alterations in mucosubstances and connective tissue were examined and their possible significance discussed. The sequence of events in healing was: 1) The mucosa adjacent to the lesion tipped into the lesioned area. The crypts in this mucosa became lined with cells which contained no mucus and had no striated borders. Later in the experimental period, these undifferentiated cells gave rise to cells containing carboxymucins. Cells containing sulfomucin, neutral mucin, or having striated borders arose from the carboxymucin cells. 2) An epithelial ledge of undifferentiated cells migrated onto a sulfated glycosaminoglycan, fibrous interface between necrotic and living tissue in the lesion. 3) Crypt formation began with the appearance of intraepithelial anlagen. 4) Crypts lengthened by a process of epithelial-connective tissue proliferation from the base of the crypt upwards. Following completion of connective tissue regeneration, crypts formed by invading the reestablished lamina propria. 5) The first mucous cells in the ledge contained carboxymucins. As crypt formation occurred, these cells gave rise to typical columnar absorptive cells, to cells containing sulfomucins, and to cells containing neutral mucins. 6) Lengthening of crypts ceased following the appearance of a sulfated acid glycosaminoglycan--collagenous layer deep in the submucosa.
作为溃疡形成与愈合研究的一部分，在大鼠身上制造手术损伤后，对结肠黏膜的再生进行了研究。检查了黏膜物质和结缔组织的变化，并讨论了它们可能的意义。愈合过程中的事件顺序如下：1）病变附近的黏膜向病变区域倾斜。该黏膜中的隐窝被不含黏液且无纹状缘的细胞所覆盖。在实验后期，这些未分化细胞产生了含有羧基黏蛋白的细胞。含有硫酸黏蛋白、中性黏蛋白或有纹状缘的细胞由羧基黏蛋白细胞产生。2）未分化细胞的上皮边缘迁移到病变处坏死组织与存活组织之间的硫酸化糖胺聚糖纤维界面上。3）隐窝形成始于上皮内胚基的出现。4）隐窝通过从隐窝底部向上的上皮 - 结缔组织增殖过程而延长。结缔组织再生完成后，隐窝通过侵入重新建立的固有层而形成。5）边缘处的第一批黏液细胞含有羧基黏蛋白。随着隐窝形成，这些细胞产生典型的柱状吸收细胞、含有硫酸黏蛋白的细胞和含有中性黏蛋白的细胞。6）黏膜下层深处出现硫酸化酸性糖胺聚糖 - 胶原层后，隐窝延长停止。
