Petersen B, Rehfeld J F
Scand J Gastroenterol. 1980;15(1):29-31. doi: 10.3109/00365528009181427.
The concentration of gastrin in serum from 47 fasting patients with duodenal ulcer was measured with two radioimmunoassays, one specific for the COOH- and the other specific for NH2-terminal sequence of gastrin-17. The COOH-terminal assay measured 13 +/- 2 pmol and the NH2-terminal 42 +/- 7 pmol-equivalent gastrin-17 per litre (mean +/- S.E.M.). This result, corroborated by gel chromatography, shows that an NH2-terminal fragment of gastrin-17, presumably the tridecapeptide, circulates in concentrations higher than those of the four known gastrin components. The abundance of NH2-terminal fragment in serum suggests that the complementary COOH-terminal tetrapeptide fragment of gastrin-17 is also produced in large quantities.
用两种放射免疫分析法测定了47例空腹十二指肠溃疡患者血清中胃泌素的浓度，一种针对胃泌素-17的COOH末端序列具有特异性，另一种针对NH2末端序列具有特异性。COOH末端分析法测得每升血清中胃泌素-17为13±2皮摩尔，NH2末端为42±7皮摩尔当量的胃泌素-17（平均值±标准误）。凝胶色谱法证实了这一结果，表明胃泌素-17的NH2末端片段，可能是十三肽，其循环浓度高于四种已知胃泌素成分的浓度。血清中NH2末端片段的丰富表明胃泌素-17的互补COOH末端四肽片段也大量产生。
