Kothary P C, Vinik A I
Biochem Biophys Res Commun. 1987 Jul 31;146(2):884-8. doi: 10.1016/0006-291x(87)90613-9.
Serum gastrin concentrations were measured using antisera with specificity for the carboxyl and amino terminus of gastrin-17 in 50 healthy subjects and 18 patients with active duodenal ulcer disease (DU). The amino terminal of gastrin-17 immunoreactivity was significantly higher in DU patients than in healthy subjects. NH2-terminus of gastrin-17 immunopurified material from serum of DU patients was subjected to Sephadex G50 column chromatography and eluates were monitored by an additional antiserum EG10 that recognizes COOH-terminally extended gastrin. Besides the NH2 terminal tridecapeptide of gastrin-17, COOH-terminally extended progastrin was found. This may reflect abnormal processing of gastrin in patients with active duodenal ulcer disease.
采用对胃泌素 - 17羧基和氨基末端具有特异性的抗血清，对50名健康受试者和18名活动性十二指肠溃疡疾病（DU）患者测定血清胃泌素浓度。DU患者中胃泌素 - 17免疫反应性的氨基末端显著高于健康受试者。对DU患者血清中免疫纯化的胃泌素 - 17物质的NH2末端进行Sephadex G50柱色谱分析，并用另一种识别COOH末端延伸胃泌素的抗血清EG10监测洗脱液。除了胃泌素 - 17的NH2末端十三肽外，还发现了COOH末端延伸的前胃泌素。这可能反映了活动性十二指肠溃疡疾病患者胃泌素的异常加工过程。
