Körbling M, Fliedner T M, Pflieger H
Scand J Haematol. 1980 Jan;24(1):22-8. doi: 10.1111/j.1600-0609.1980.tb01313.x.
Continuous-flow leukapheresis using the AMINCO Celltrifuge was performed in 35 adult volunteers for the procurement of large quantities of granulocyte/macrophage progenitor cells (CFUc) as part of the mononuclear leucocytes. The donor blood volume processed was 12 1 at a flow rate of 50 ml per min. The centrifuge speed was 800 rpm. The mean yield of mononuclear cells (MNC) per run was 12.0 x 10(9) and that of CFUc, 8.7 x 10(5). The leukapheresis did not much affect the donor blood cell concentration except the platelet concentration; this must be considered as a limiting factor for leukapheresis. The mean granulocyte contamination of the leukapheresis-derived leucocytes was only about 10%. Successive leukaphereses (with short-term intervals) in 7 donors led to significant increases in CFUc yield. The possible therapeutic application of blood stem cells in the treatment of haemopoietic failure in man is discussed.
使用AMINCO血细胞分离机进行连续流式白细胞去除术，对35名成年志愿者进行操作，以获取大量作为单核白细胞一部分的粒细胞/巨噬细胞祖细胞（CFUc）。处理的供体血量为12升，流速为每分钟50毫升。离心机速度为800转/分钟。每次运行的单核细胞（MNC）平均产量为12.0×10⁹，CFUc的平均产量为8.7×10⁵。白细胞去除术对供体血细胞浓度影响不大，除了血小板浓度；这必须被视为白细胞去除术的一个限制因素。白细胞去除术获得的白细胞中粒细胞的平均污染率仅约为10%。7名供体连续进行白细胞去除术（间隔时间短）导致CFUc产量显著增加。讨论了血液干细胞在治疗人类造血功能衰竭中的可能治疗应用。
