Kovács P, Bruch C, Herbst E W, Fliedner T M
Acta Haematol. 1978;60(3):172-81. doi: 10.1159/000207713.
The presence of in vitro colony-forming units (CFUc) was demonstrated in leukocyte suspensions collected by continuous flow leukaphereses performed serially in dogs. The CFUc yield of one leukapheresis was usually between 10(4) and 10(6). Higher yields were obtained in the 2nd to 5th leukaphereses than in the first one in the same dog. The number of CFUc collected during one leukapheresis was about four times the number of CFUc present in the circulating blood at the beginning of the leukapheresis. After a diminution during leukapheresis the circulating CFUc pool was restored in 2 or 3 days with an increase in the ratio of CFUc to mononuclear cells. These results indicate that CFUc entered the circulating pool during and after leukapheresis and that the population size regulation of canine-circulating CFUc is different from that of the bulk of the mononuclear leukoytes.
在通过连续流动白细胞单采术连续采集的犬白细胞悬液中，证实了体外集落形成单位（CFUc）的存在。一次白细胞单采术的CFUc产量通常在10⁴至10⁶之间。同一只犬第二次至第五次白细胞单采术的产量高于第一次。一次白细胞单采术中收集的CFUc数量约为白细胞单采术开始时循环血液中CFUc数量的四倍。白细胞单采术期间CFUc数量减少后，循环CFUc池在2至3天内恢复，CFUc与单核细胞的比例增加。这些结果表明，CFUc在白细胞单采术期间及之后进入循环池，并且犬循环CFUc的群体大小调节与大多数单核白细胞不同。
