Shostak I N, Rozhko O T, Martynenko F P
Vopr Med Khim. 1980 Jan-Feb;26(1):71-5.
Definite separation of somatotropin and prolactin using sodium dodecylsulphate polyacrylamide gel electrophoresis takes place in continuous systems with pH 7.0-7.2 containing acetate or chloride monovalet anions; substitution of the ions by polyvalent phosphate or borate anions interfered with the separation. The hormones precipitated by trichloracetic acid may be separated and quantitatively determined after dissolving in the weak alkaline solutions. The hormones are distinctly separated from serum proteins and may be quantitatively determined in media containing up to 15% of serum.
使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳对生长激素和催乳素进行明确分离，是在含有醋酸盐或氯化物单价阴离子、pH值为7.0 - 7.2的连续系统中进行的；用多价磷酸盐或硼酸盐阴离子替代这些离子会干扰分离。用三氯乙酸沉淀的激素在溶解于弱碱性溶液后可进行分离和定量测定。这些激素能与血清蛋白明显分离，并且在含有高达15%血清的介质中可进行定量测定。
