Rogol A D, Grissom F, Follows R E
Horm Metab Res. 1978 Nov;10(6):515-20. doi: 10.1055/s-0028-1093382.
The bovine diabetogenic peptide (Louis) has been further purified by gel filtration. Material from the major protein peak was submitted to quantitative polyacrylamide gel electrophoresis. By the criteria of the joint 95 percent confidence envelope of KR and YO, related to molecular size and net charge, the diabetogenic peptide more closely resembles prolactin than growth hormone. By sodium dodecyl sulfate polyacrylamide gel electrophoresis, bovine diabetogenic peptide has a molecular weight of 24.000 daltons, slightly greater than that for prolactin. Amino acid analysis has revealed remarkable similarity between the diabetogenic peptide and prolactin; particularly in the numbers of residues of 1/2 cystine, proline, histidine and phenylalanine. The amino terminal amino acid sequence is methionyl-phenylalanyl-phenylalanine. By the criteria of molecular size, quantitative polyacrylamide gel electrophoresis, amino acid composition and amino terminal sequence analysis, we consider the relationship between the diabetogenic peptide and prolactin.
牛致糖尿病肽（路易斯）已通过凝胶过滤进一步纯化。来自主要蛋白质峰的物质进行了定量聚丙烯酰胺凝胶电泳。根据与分子大小和净电荷相关的KR和YO的联合95%置信区间标准，致糖尿病肽与催乳素的相似性比生长激素更高。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳，牛致糖尿病肽的分子量为24000道尔顿，略大于催乳素。氨基酸分析显示致糖尿病肽与催乳素之间存在显著相似性；特别是在半胱氨酸、脯氨酸、组氨酸和苯丙氨酸残基的数量上。氨基末端氨基酸序列为甲硫氨酰-苯丙氨酰-苯丙氨酸。根据分子大小、定量聚丙烯酰胺凝胶电泳、氨基酸组成和氨基末端序列分析标准，我们考虑了致糖尿病肽与催乳素之间的关系。
