衣霉素对正常及低密度脂蛋白受体缺陷的人皮肤成纤维细胞代谢低密度脂蛋白的影响
Effect of tunicamycin on the metabolism of low-density lipoproteins by control and low-density-lipoprotein-receptor-deficient human skin fibroblasts.
作者信息
Filipovic I, von Figura K
出版信息
Biochem J. 1980 Jan 15;186(1):373-5. doi: 10.1042/bj1860373.
Abstract
Preincubation of normal human skin fibroblasts with tunicamycin, which inhibits N-glycosylation of glycoproteins, resulted in a dose-dependent and reversible inhibition of binding and internalization of homologous low-density lipoproteins by the cells. The degradation of the internalized lipoproteins was not affected by the drug. Comparative studies with fibroblasts deficient in low-density-lipoprotein receptors indicated that tunicamycin exerts its inhibitory effect only via the receptor-mediated high-affinity binding and uptake of lipoproteins. These results suggest that expression of low-density-lipoprotein receptors on the cell surface of human skin fibroblasts depends on intact N-glycosylation.
摘要
用衣霉素（它抑制糖蛋白的N-糖基化）对正常人皮肤成纤维细胞进行预孵育，导致细胞对同源低密度脂蛋白的结合和内化受到剂量依赖性且可逆的抑制。内化脂蛋白的降解不受该药物影响。对缺乏低密度脂蛋白受体的成纤维细胞进行的比较研究表明，衣霉素仅通过受体介导的脂蛋白高亲和力结合和摄取发挥其抑制作用。这些结果表明，人皮肤成纤维细胞表面低密度脂蛋白受体的表达取决于完整的N-糖基化。
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