Filipovic I, Schwarzmann G, Mraz W, Wiegandt H, Buddecke E
Eur J Biochem. 1979 Jan 2;93(1):51-5. doi: 10.1111/j.1432-1033.1979.tb12793.x.
The (high-affinity receptor)-mediated uptake of homologous low-density (low-rho) lipoproteins by cultured human arterial smooth muscle cells or human skin fibroblasts is controlled by the sialic acid content of low-rho lipoprotein particles. This conclusion is derived from the following results. 1. Gangliosides incubated with native low-rho lipoproteins associate with low-rho lipoprotein particles. Low-rho lipoproteins modified by associated GLac1, GGtet1, and GGtet2b + GGtet3 gangliosides are internalized by arterial smooth muscle cells at a rate up to 80% lower than native low-rho lipoproteins or those preincubated with desialized gangliosides. 2. The inhibitory effect of gangliosides is specific for high affinity uptake and not detectable on skin fibroblasts deficient in low-rho-lipoprotein receptor. 3. Desialyzed low-rho lipoproteins are internalized by smooth muscle cells up to 100% faster than native low-rho lipoproteins, the enhancement of uptake corresponding to the degree of desialization.
培养的人动脉平滑肌细胞或人皮肤成纤维细胞通过（高亲和力受体）介导摄取同源低密度（低rho）脂蛋白，这一过程受低rho脂蛋白颗粒中唾液酸含量的控制。这一结论源于以下结果。1. 与天然低rho脂蛋白一起孵育的神经节苷脂会与低rho脂蛋白颗粒结合。经相关的GLac1、GGtet1和GGtet2b + GGtet3神经节苷脂修饰的低rho脂蛋白被动脉平滑肌细胞内化的速率比天然低rho脂蛋白或与去唾液酸化神经节苷脂预孵育的低rho脂蛋白低80%。2. 神经节苷脂的抑制作用对高亲和力摄取具有特异性，在缺乏低rho脂蛋白受体的皮肤成纤维细胞上无法检测到。3. 去唾液酸化的低rho脂蛋白被平滑肌细胞内化的速度比天然低rho脂蛋白快100%，摄取的增强程度与去唾液酸化程度相对应。
