Monaselidze D R, Chanchalashvili Z I, Mgeladze G N, Madzhgaladze G V, Chitadze G Sh
Biofizika. 1980 Jan-Feb;25(1):174-6.
It has been shown that melting of native chromatin complex in tissues and cells is a one stage process with transition parameters Tm = 82 +/- 1 degrees C, delta Tm = 6 degrees C and Qm = (24 +/- 3) cal/g DNA. A conclusion is made that the difference in melting temperatures of chromatin complex and DNA, which is 12 cal/g DNA, corresponds to the melting of secondary and tertiary structures of chromatin. It is shown the destruction of significant part of the secondary and tertiary structures of chromatin by the chromatin complex isolation from tissues and cells.
已经表明，组织和细胞中天然染色质复合物的解链是一个单阶段过程，其转变参数为Tm = 82±1℃，ΔTm = 6℃，Qm =（24±3）cal/g DNA。得出的结论是，染色质复合物和DNA解链温度的差异为12 cal/g DNA，这对应于染色质二级和三级结构的解链。结果表明，通过从组织和细胞中分离染色质复合物，染色质的二级和三级结构的很大一部分被破坏。
