大鼠膀胱原位冷冻：通过³²P后标记法检测膀胱上皮细胞中的DNA加合物形成。
In situ freezing of the rat urinary bladder: DNA adduct formation in the bladder epithelium demonstrated by 32P-postlabeling assay.
作者信息
Hasegawa R, Yamashita K, Morimoto K, Furukawa F, Toyoda K, Shimoji N, Takahashi M, Hayashi Y
机构信息
Division of Pathology, National Institute of Hygienic Sciences, Tokyo.
出版信息
Jpn J Cancer Res. 1989 Sep;80(9):826-32. doi: 10.1111/j.1349-7006.1989.tb01722.x.
In situ freezing of the urinary bladder has been demonstrated to exert tumor-initiating potential in two-stage urinary bladder carcinogenesis in the rat. In the present experiment, DNA modification was examined after in situ freezing of the whole urinary bladder performed by pinching with frozen forceps at -15 degrees C or -30 degrees C for 2 s. The 32P-postlabeling analysis revealed at least 2 DNA adducts in the epithelial cells of the urinary bladder collected 3 days after freezing. Single-strand breaks of DNA were also found by means of the alkaline elution assay in the bladder epithelium collected 10 min after freezing. Thus, the previously demonstrated tumor-initiating activity of in situ freezing in urinary bladder carcinogenesis was revealed to be associated with substantial DNA damage and adduct formation.
在大鼠膀胱的两阶段致癌过程中，原位冷冻膀胱已被证明具有引发肿瘤的潜力。在本实验中，通过在-15℃或-30℃下用冷冻镊子夹捏整个膀胱2秒进行原位冷冻后，检测DNA修饰情况。32P后标记分析显示，冷冻后3天收集的膀胱上皮细胞中至少有2种DNA加合物。通过碱性洗脱试验还发现，冷冻后10分钟收集的膀胱上皮细胞中存在DNA单链断裂。因此，先前证明的原位冷冻在膀胱癌发生中的肿瘤引发活性被发现与大量DNA损伤和加合物形成有关。
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