Kjeld J M, Wieland J M, Puah M
Clin Chim Acta. 1980 Mar 28;102(2-3):119-26. doi: 10.1016/0009-8981(80)90024-8.
The discovery of two previously unknown progesterone conjugates in human urine is presented. Urine from near-term pregnant women was applied to an anion exchange column and then eluted with an increasing concentration gradient of sodium chloride. Aliquots of collected fractions were subjected to four different treatments: (1) immediate extraction, (2) incubation at 37 degrees C, (3) beta-glucuronidase hydrolysis and (4) acid hydrolysis. All eluent aliquots were then further purified on a thin layer chromatographic system and measured for progesterone content by radioimmunoassay. The results suggest that progesterone, a non-hydroxylated delta 4-3 ketosteroid, occurs in at least two negatively charged conjugated forms in human urine. Comparison with elution patterns of known testosterone conjugates shows that these conjugates of progesterone travel on an anion exchange column similarly to testosterone glucuronide and testosterone sulphate. Although the exact structure of these progesterone conjugates remains to be elucidated, the property of physiological conjugation of progesterone has not previously been described. Quantitatively each of these conjugates appears to exist in amounts equal to or greater than the levels of free (unconjugated) urinary progesterone.
本文介绍了在人尿中发现两种此前未知的孕酮结合物。将足月孕妇的尿液应用于阴离子交换柱，然后用氯化钠浓度递增的梯度洗脱。收集的馏分等分试样进行四种不同处理：(1)立即萃取，(2)在37℃孵育，(3)β-葡萄糖醛酸酶水解和(4)酸水解。然后将所有洗脱液等分试样在薄层层析系统上进一步纯化，并通过放射免疫测定法测定孕酮含量。结果表明，孕酮作为一种非羟基化的δ4-3酮类固醇，在人尿中至少以两种带负电荷的结合形式存在。与已知睾酮结合物的洗脱模式比较表明，这些孕酮结合物在阴离子交换柱上的移动方式与睾酮葡萄糖醛酸酯和睾酮硫酸盐相似。虽然这些孕酮结合物的确切结构仍有待阐明，但孕酮的生理结合特性此前尚未有描述。定量分析表明，每种结合物的存在量似乎等于或大于游离（未结合）尿孕酮的水平。
