Puah C M, Kjeld J M, Joplin G F
J Chromatogr. 1978 Mar 1;145(2):247-55. doi: 10.1016/s0378-4347(00)81345-6.
A method for the analysis of testosterone (and 5alpha-dihydrotestosterone) conjugates in human serum and urine samples is described. The samples were grought to pH 1 and extracted with a diethyl ether-methanol mixture. After evaporation the residues were run in a thin-layer chromatography system, individual samples' paths were cut into 1-cm long pieces and eluted with methanol. The methanol was evaporated and the residue subjected to acid hydrolysis. The released steroid was extracted by diethyl ether and measured by radioimmunoassay. The methodology described represents a new approach to the qualitative and quantitative study of steroid conjugates in serum and urine, and can easily be applied to the study of steroid conjugates in other biological mateiral.
描述了一种分析人血清和尿液样本中睾酮（及5α-二氢睾酮）结合物的方法。将样本调至pH 1，并用乙醚 - 甲醇混合物萃取。蒸发后，将残留物在薄层色谱系统中展开，将各个样本的条带切成1厘米长的片段，并用甲醇洗脱。蒸干甲醇，残留物进行酸水解。释放出的类固醇用乙醚萃取并用放射免疫分析法测定。所描述的方法代表了一种用于血清和尿液中类固醇结合物定性和定量研究的新方法，并且可以很容易地应用于其他生物材料中类固醇结合物的研究。
