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Is the thiolate--imidazolium ion pair the catalytically important form of papain?

作者信息

Creighton D J, Gessouroun M S, Heapes J M

出版信息

FEBS Lett. 1980 Feb 11;110(2):319-22. doi: 10.1016/0014-5793(80)80101-3.

DOI:10.1016/0014-5793(80)80101-3
PMID:7371836
Abstract
摘要

相似文献

1
Is the thiolate--imidazolium ion pair the catalytically important form of papain?硫醇盐 - 咪唑鎓离子对是木瓜蛋白酶具有催化活性的重要形式吗？
FEBS Lett. 1980 Feb 11;110(2):319-22. doi: 10.1016/0014-5793(80)80101-3.
2
Ionization of a nitrophenol-containing reporter group at the active site of papain.木瓜蛋白酶活性位点上含硝基苯酚的报告基团的电离作用。
Biochemistry. 1974 Feb 12;13(4):690-8. doi: 10.1021/bi00701a009.
3
On the mode of activation of the catalytically essential sulfhydryl group of papain.关于木瓜蛋白酶催化必需巯基的激活模式。
Eur J Biochem. 1973 Feb 15;33(1):104-9. doi: 10.1111/j.1432-1033.1973.tb02660.x.
4
Effect of hydroxynitrobenzylation of tryptophan-177 on reactivity of active site cysteine-25 in papain.
Arch Biochem Biophys. 1981 Feb;206(2):362-71. doi: 10.1016/0003-9861(81)90103-x.
5
Investigation of the catalytic site of actinidin by using benzofuroxan as a reactivity probe with selectivity for the thiolate-imidazolium ion-pair systems of cysteine proteinases. Evidence that the reaction of the ion-pair of actinidin (pKI 3.0, pKII 9.6) is modulated by the state of ionization of a group associated with a molecular pKa of 5.5.以苯并呋咱为反应性探针研究猕猴桃蛋白酶的催化位点，该探针可选择性作用于半胱氨酸蛋白酶的硫醇盐-咪唑鎓离子对体系。有证据表明，猕猴桃蛋白酶（pKI 3.0，pKII 9.6）离子对的反应受一个与分子pKa为5.5的基团的电离状态调节。
Biochem J. 1983 Sep 1;213(3):713-8. doi: 10.1042/bj2130713.
6
Evidence for a close similarity in the catalytic sites of papain and ficin in near-neutral media despite differences in acidic and alkaline media. Kinetics of the reactions of papain and ficin with chloroacetate.尽管在酸性和碱性介质中存在差异，但木瓜蛋白酶和无花果蛋白酶在近中性介质中的催化位点具有高度相似性的证据。木瓜蛋白酶和无花果蛋白酶与氯乙酸反应的动力学。
Biochem J. 1982 Jan 1;201(1):101-4. doi: 10.1042/bj2010101.
7
Proton equilibria in the binding of Zn2+ and of methylmercuric iodide to papain.
Eur J Biochem. 1976 Dec 11;71(2):383-91. doi: 10.1111/j.1432-1033.1976.tb11125.x.
8
2-Chloromethyl-4-nitrophenyl (N-carbobenzoxy)glycinate. A new reagent designed to introduce an environmentally sensitive conformational probe near the active site of papain.2-氯甲基-4-硝基苯基（N-苄氧羰基）甘氨酸酯。一种旨在在木瓜蛋白酶活性位点附近引入环境敏感构象探针的新型试剂。
Biochemistry. 1973 Dec 18;12(26):5278-85. doi: 10.1021/bi00750a009.
9
Consequences of molecular recognition in the S1-S2 intersubsite region of papain for catalytic-site chemistry. Change in pH-dependence characteristics and generation of an inverse solvent kinetic isotope effect by introduction of a P1-P2 amide bond into a two-protonic-state reactivity probe.木瓜蛋白酶S1 - S2亚位点间区域分子识别对催化位点化学的影响。通过将P1 - P2酰胺键引入双质子态反应性探针，pH依赖性特征的变化及反向溶剂动力学同位素效应的产生。
Biochem J. 1988 Mar 15;250(3):761-72. doi: 10.1042/bj2500761.
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Mercaptide-imidazolium ion-pair: the reactive nucleophile in papain catalysis.硫醇盐 - 咪唑鎓离子对：木瓜蛋白酶催化中的活性亲核试剂。
FEBS Lett. 1974 Oct 1;47(1):15-8. doi: 10.1016/0014-5793(74)80415-1.

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