Poeggel G, Bernstein H G, Luppa H
Histochemistry. 1980;65(3):315-19. doi: 10.1007/BF00493181.
The fate of ATP exposed to rat hippocampal extracts was investigated after their separation by a microdisc electrophoresis tenhnique. It could be demonstrated that the histochemical adenylate cyclase procedure using ATP as substrate is not suitable for specific localization of the enzyme, since other ATP hydrolysing enzymes were also able to convert ATP unless the concentrations of inhibitors reached 1 mM (ouabain) and 40 mM (NaF). With a prolonged incubation time of 18 h further substrate splitting protein zones could be revealed, possibly reflecting activities of enzymes involved in the hydrolysis of degradation products of ATP.
通过微量盘电泳技术分离后，研究了暴露于大鼠海马提取物中的ATP的命运。可以证明，以ATP为底物的组织化学腺苷酸环化酶方法不适用于该酶的特异性定位，因为除非抑制剂浓度达到1 mM（哇巴因）和40 mM（氟化钠），其他ATP水解酶也能够转化ATP。随着孵育时间延长至18小时，可揭示出更多的底物分解蛋白区带，这可能反映了参与ATP降解产物水解的酶的活性。
