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嗜酸氧化亚铁硫杆菌囊泡中通过与环境条件相当的H⁺离子梯度形成腺苷5'-三磷酸。

Adenosine 5'-triphosphate formation in Thiobacillus ferrooxidans vesicles by H+ ion gradients comparable to those of environmental conditions.

作者信息

Apel W A, Dugan P R, Tuttle J H

出版信息

J Bacteriol. 1980 Apr;142(1):295-301. doi: 10.1128/jb.142.1.295-301.1980.

DOI:10.1128/jb.142.1.295-301.1980
PMID:7372573
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC293951/
Abstract

Vesicles prepared from iron-grown Thiobacillus ferrooxidans, and subsequently loaded with adenosine 5'-diphosphate and inorganic phosphate, produced adenosine 5'-triphosphate when subjected to H+ gradients comparable to those in the cells' normal environment (i.e., an internal pH in the range of 6.0 to 8.0 with an optimum of 7.0 to 7.8 and an external pH in the range of 2.1 to 4.1 with an optimum of 2.8). Nigericin, dicyclohexylcarbodiimide, and pentachlorophenol decreased adenosine 5'-triphosphate synthesis. Valinomycin at concentrations of 2.5 and 5.0 micrograms/ml increased adenosine 5'-triphosphate formation by 25 and 30%, respectively.

摘要

由铁营养型氧化亚铁硫杆菌制备的囊泡，随后装载腺苷5'-二磷酸和无机磷酸，当受到与细胞正常环境中相当的H+梯度（即内部pH值在6.0至8.0范围内，最适值为7.0至7.8，外部pH值在2.1至4.1范围内，最适值为2.8）作用时，会产生腺苷5'-三磷酸。尼日利亚菌素、二环己基碳二亚胺和五氯苯酚会降低腺苷5'-三磷酸的合成。浓度为2.5和5.0微克/毫升的缬氨霉素分别使腺苷5'-三磷酸的形成增加了25%和30%。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1b3/293951/bb0f442c2281/jbacter00565-0312-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1b3/293951/8943d000a4a1/jbacter00565-0310-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1b3/293951/bb0f442c2281/jbacter00565-0312-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1b3/293951/8943d000a4a1/jbacter00565-0310-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1b3/293951/bb0f442c2281/jbacter00565-0312-a.jpg

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本文引用的文献

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