Buckingham R B, Prince R K, Rodnan G P, Barnes E L
J Rheumatol. 1980 Mar-Apr;7(2):130-42.
Two patients with linear localized scleroderma are reported. Punch biopsies from involved and contralateral site-matched uninvolved dermis of each patient were explained for the purpose of studying connective tissue accumulation in fibroblast monolayer culture. Monolayer cultures from involved tissue accumulated more collagen and glycosaminoglycan than cultures from uninvolved tissue for both patients. Augmented accumulation of collagen persisted for at least 11 subpassages. Involved monolayers acquired a strikingly different macroscopic appearance in confluent cultures.
报告了两名线性局限性硬皮病患者。为了研究成纤维细胞单层培养中结缔组织的积累情况，对每位患者受累皮肤及对侧部位匹配的未受累真皮进行了钻孔活检。两名患者受累组织的单层培养物比未受累组织的培养物积累了更多的胶原蛋白和糖胺聚糖。胶原蛋白的积累增加至少持续了11代传代培养。在汇合培养中，受累的单层细胞呈现出明显不同的宏观外观。
