McArthur W, Yaari A M, Shapiro I M
Lab Invest. 1980 Apr;42(4):450-6.
Mononuclear cells derived from chicken peripheral blood or from thioglycollate-induced mouse peritoneal exudates were found to cause calcium release from devitalized homologous bone in vitro. These mononuclear cells with osteolytic activity were adherent to plastic surfaces and were identified as being macrophages by cell surface markers and histochemical staining. Other mononuclear cells such as chicken thymocytes, nonadherent peripheral blood mononuclear cells, and chick embryo fibroblasts did not cause bone dissolution. In parallel with the active solubilization of bone mineral, 14C-label was also released from devitalized calvaria prelabeled with 14C-proline. Macrophages, inactivated by repeated freezing and thawing as well as those cultured in the presence of iodoacetate, did not solubilize bone in vitro. The degree of bone solubilization was directly related to the numbers of macrophages per culture as well as the duration of the culture period. Powdered devitalized homologous bone was used in most experiments, but macrophages were also able to solubilize bone material in vitro from devitalized calvaria and bone slabs. The addition of Escherichia coli lipopolysaccharide to cultures of bone and macrophages significantly increased the levels of calcium released from bone. The addition of parathyroid hormone and calcitonin had no effect on macrophage-mediated bone dissolution. These results suggest that viable macrophages have osteolytic activity and that this activity is modulated by an inflammatory mediator, endotoxin.
研究发现，源自鸡外周血或巯基乙酸盐诱导的小鼠腹腔渗出液的单核细胞，在体外可导致失活的同源骨释放钙。这些具有溶骨活性的单核细胞能黏附于塑料表面，通过细胞表面标志物和组织化学染色鉴定为巨噬细胞。其他单核细胞，如鸡胸腺细胞、非黏附性外周血单核细胞和鸡胚成纤维细胞，则不会引起骨溶解。与骨矿物质的活性溶解同时发生的是，预先用¹⁴C - 脯氨酸标记的失活颅盖骨也释放出¹⁴C标记物。经反复冻融灭活的巨噬细胞以及在碘乙酸存在下培养的巨噬细胞，在体外均不能溶解骨。骨溶解的程度与每个培养物中的巨噬细胞数量以及培养时间直接相关。大多数实验使用的是粉末状失活同源骨，但巨噬细胞也能够在体外溶解来自失活颅盖骨和骨板的骨材料。向骨和巨噬细胞培养物中添加大肠杆菌脂多糖，可显著增加从骨中释放的钙水平。添加甲状旁腺激素和降钙素对巨噬细胞介导的骨溶解没有影响。这些结果表明，活巨噬细胞具有溶骨活性，且这种活性受炎症介质内毒素的调节。
