Tsao S W, Burman J F, Easty D M, Easty G C, Carter R L
Br J Cancer. 1981 Mar;43(3):392-401. doi: 10.1038/bjc.1981.60.
An in vitro osteolysis assay with 45Ca-labelled mouse calvaria has been used to investigate mechanisms of direct bone invasion by squamous carcinomas of the head and neck. Short-term (3-day) organ cultures of 8 fresh squamous carcinomas showed varying degrees of in vitro bone-resorbing activity which was blocked by indomethacin, an inhibitor of prostaglandin synthesis. Supernatant media from 6 established cell lines also induced bone resorption in vitro and evoked an osteoclastic response in the cultured calvaria. Osteolysis by supernatant media was not blocked by indomethacin in all the tumour-cell lines, and the production of non-prostaglandin osteolysins by the indomethacin-resistant lines is postulated. The two principal findings that emerge are: (1) Stimulants for osteoclastic activity are derived from both squamous-carcinoma cells and from host cells in the tumour stroma. (2) These stimulants are diverse. Indomethacin-sensitive agents, presumed to be prostaglandins, are most convincingly demonstrated in the fresh tumours. Indomethacin-resistant agents, presumably not prostaglandins, are more characteristic of the carcinoma cell lines.
利用45Ca标记的小鼠颅骨进行的体外骨溶解试验，已被用于研究头颈部鳞状细胞癌直接侵犯骨骼的机制。对8例新鲜鳞状细胞癌进行的短期（3天）器官培养显示出不同程度的体外骨吸收活性，这种活性被前列腺素合成抑制剂吲哚美辛所阻断。来自6个已建立的细胞系的上清培养基在体外也诱导了骨吸收，并在培养的颅骨中引发了破骨细胞反应。上清培养基引起的骨溶解在所有肿瘤细胞系中均未被吲哚美辛阻断，推测吲哚美辛耐药系会产生非前列腺素类骨溶解素。得出的两个主要发现是：（1）破骨细胞活性的刺激物既来源于鳞状癌细胞，也来源于肿瘤基质中的宿主细胞。（2）这些刺激物是多种多样的。在新鲜肿瘤中最有说服力地证明了对吲哚美辛敏感的物质，推测为前列腺素。对吲哚美辛耐药的物质，大概不是前列腺素，更具癌细胞系的特征。
